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P0914

Sigma-Aldrich

Protein standard

Micro Standard, liquid

Sinónimos:

Albumin from bovine serum, protein standard

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.27
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

bovine

Nivel de calidad

Formulario

liquid

contiene

0.05% sodium azide as preservative

envase

ampule of 1 mL

concentración

0.98—1.02 mg protein/mL

técnicas

ELISA: suitable

color

clear colorless

Nº de acceso UniProt

temp. de almacenamiento

2-8°C

Información sobre el gen

bovine ... ALB(280717)

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Aplicación

Concentrated solution for all protein assays.
Protein standard has been used:

  • as a standard in the quantification of protein release by bicinchoninic acid assay[1]
  • as a standard in the quantification of urea by Bradfordassay[1]
  • as a molecular size marker for the quantification of protein by native polyacrylamide gel electrophoresis[2]
Protein standard for all protein assays. The sealed ampules provide assurance that the concentration will be correct with every assay.

Envase

Sealed ampules

Otras notas

1 mg bovine serum albumin/ml in 0.15 M NaCl

Idoneidad

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

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Alexander Pekarsky et al.
Bioprocess and biosystems engineering, 42(10), 1611-1624 (2019-07-04)
In biotechnological processes, technical failures in the upstream process often lead to batch loss. It is of great interest to investigate the empirical impact of technical failures to understand and mitigate their impact accurately and reduce economic damage. We investigated
Screening and characterization of a novel esterase from a metagenomic library
Yun-Jung K, et al.
Protein Expression and Purification, 45(2), 315-323 (2006)
Mudalige Don Hiranya Jayasanka Senavirathna et al.
Water environment research : a research publication of the Water Environment Federation, 95(1), e10834-e10834 (2023-01-13)
This study demonstrated the temporal variation of 2-methylisoborneol (2-MIB) and geosmin (GSM) production of two filamentous cyanobacteria species Pseudanabaena galeata (NIES-512; planktonic) and Phormidium ambiguum (NIES-2119; benthic) exposed to high light intensity (950-1000 μmol m-2  s-1 photosynthetically active radiation). The production of
Alexander Pekarsky et al.
Bioprocess and biosystems engineering, 44(6), 1049-1061 (2021-01-26)
Technical failures lead to deviations in process parameters that can exceed studied process boundaries. The impact on cell and target protein is often unknown. However, investigations on common technical failures might yield interesting insights into process and protein robustness. Recently
Shouliang Xiong et al.
Bioengineered, 12(2), 11138-11155 (2021-11-17)
Osteoarthritis (OA) is characterized by destruction of articular cartilage with an imbalance between synthesis and degradation of extracellular matrix (ECM). In the current study, we explored the role of microRNA-34a (miR-34a) and the behind epigenetic mechanism in the degradation of

Protocolos

To standardize a procedure for the determination of protein by modified Lowry.

To standardize a procedure for the determination of protein by modified Lowry.

To standardize a procedure for the determination of protein by modified Lowry.

To standardize a procedure for the determination of protein by modified Lowry.

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Contenido relacionado

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Métodos de cuantificación de proteínas, reactivos y tecnología de inmunoanálisis para la medición precisa de las concentraciones de proteínas en una variedad de muestras.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Ver todo

Questions

1–4 of 4 Questions  
  1. Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?

    1 answer
    1. This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.

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  2. What is the shipping temperature?

    1 answer
    1. The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?

    1 answer
    1. The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).

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