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Key Documents

43925

Sigma-Aldrich

Nigrosin Stain solution

for microscopy

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

Bacillus spp.
Corynebacterium spp.

General description

Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.

Components

Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water

pictograms

Health hazardExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves


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R R Valle et al.
Theriogenology, 70(1), 115-120 (2008-05-16)
Simple, rapid and stable sperm evaluation methods which have been optimized for common marmoset (Callithrix jacchus) are critical for studies involving collection and evaluation of sperm in the field. This is particularly important for new species groups such as Callitrichidae
Emmanuel I Odongo-Aginya et al.
African health sciences, 7(1), 33-36 (2007-07-03)
The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the
S C Esteves et al.
Fertility and sterility, 66(5), 798-804 (1996-11-01)
To determine whether the hypo-osmotic swelling test can assess viability of frozen spermatozoa. Fresh semen specimens were divided into two aliquots: one was not processed and the other was processed by swim-up. Both aliquots were then cryopreserved by the liquid
A Novelli
Pathologica, 88(3), 188-191 (1996-06-01)
Nigrosin, an aqueous blue-black acid dye of the azine series, in conjunction with Biebrich scarlet, orange G and formic acid as mordant, it is found an excellent triple panchromatic rapid stain for histological purposes. The staining so obtained permits a
L Björndahl et al.
Human reproduction (Oxford, England), 18(4), 813-816 (2003-03-28)
The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. The technique was

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