12158167001
Roche
Anti-HA-Biotin, High Affinity (3F10)
from rat IgG1
Sinónimos:
antibody
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About This Item
Código UNSPSC:
12352203
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
rat
Nivel de calidad
conjugado
biotin conjugate
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
3F10, monoclonal
Formulario
lyophilized (stabilized)
envase
pkg of 50 μg
fabricante / nombre comercial
Roche
isotipo
IgG1
secuencia del epítopo
YPYDVPDYA
temp. de almacenamiento
2-8°C
Categorías relacionadas
Descripción general
Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins.
Especificidad
Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Inmunógeno
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Aplicación
Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using:
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.[1][2][3]
- Dot blots
- ELISA (enzyme-linked immunosorbent assay)
- Western blots
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.[1][2][3]
Calidad
Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein.
Nota de preparación
Sample Materials
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
- Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw.
- Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets.
- Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix:
- Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications.
- Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis.
Working concentration: Working concentration of conjugate depends on application and substrate
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 100 ng/ml
- ELISA: 100 ng/ml
- Western blot: 100 ng/ml
Reconstitución
Add 1 ml double-distilled water to a final concentration of 50 μg/ml.
Rehydrate for 10 minutes prior to use.
Rehydrate for 10 minutes prior to use.
Otras notas
For life science research only. Not for use in diagnostic procedures.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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