11796895001
Roche
DIG Easy Hyb™ Granules
powder, suitable for hybridization
Sinónimos:
DIG system
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About This Item
UNSPSC Code:
41105326
Productos recomendados
agency
suitable for
Quality Level
form
powder
usage
sufficient for 6 x 100 mL
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
hybridization: suitable
color
white
pH
7,0
solubility
water: soluble
suitability
suitable for nucleic acid hybridization
greener alternative category
storage temp.
20-25°C
General description
DIG Easy Hyb™ is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.
Features and Benefits
- DIG Easy Hyb™ is a convenient ready-to-use hybridization buffer, saving time and effort.
- DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.
Preparation Note
Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
DIG Easy Hyb is a trademark of Roche
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting
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