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Merck

11299964001

Roche

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II

sufficient for ≤100 tests, storage temp.:-20°C

Sinónimos:

5-BrdU, 5-Bromo-2-deoxyuridine

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About This Item

UNSPSC Code:
41116133

Quality Level

usage

sufficient for ≤100 tests

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.

Specificity

Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Application

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II has been used in:
  • labeling of tooth roots for histology
  • immunostaining of mice frontal sections
  • immunofluorescence imaging of hepatocellular carcinoma sections

The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.

Features and Benefits

  • Safe: No radioisotopes are used.
  • Easy to perform: Follows a standard immunohistochemistry protocol.
  • Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
  • Flexible: Allows double-labeling protocols.

Packaging

1 kit containing 7 components.

Principle

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.

Preparation Note

Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture:
adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Solo componentes del kit

Referencia del producto
Descripción

  • BrdU Labeling Reagent 1,000x concentrated

  • Washing Buffer concentrate 10x concentrated

  • Incubation Buffer

  • Anti-BrdU antibody, contains nucleases for DNA denaturation

  • Anti-mouse Ig-alkaline Phosphatase antibody

  • NBT

  • BCIP

signalword

Danger

Hazard Classifications

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Muta. 1B - Repr. 1B - Skin Sens. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

136.4 °F

flash_point_c

58 °C


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Artículos

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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