MABS1327

Anti-Podocalyxin (GP135) Antibody, clone 3F2:D8

clone 3F2:D8, from mouse

• Sinónimos: Podocalyxin-like protein 1, GP1345, PC, PC-like protein 1, PCLP-1, cPCLP1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3F2:D8, monoclonal
• reactividad de especies: canine
• envase: antibody small pack of 25 μL
• técnicas: immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; radioimmunoassay: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001018645
• Nº de acceso UniProt: Q52S86
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PODXL(5420)

Descripción

Descripción general

Podocalyxin (UniProt: Q52S86; also known as Gp135, Podocalyxin-like protein 1, PC, PC-like protein 1, PCLP-1, cPCLP1) is encoded by the PODXL (also known as PCLP, PCLP1) gene (Gene ID: 482252) in canine species. GP135 is an apical membrane protein expressed in polarized MDCK epithelial cells. Some expression is also observed in heart, lung, renal cortex and medulla, and muscle. It has an extracellular domain (aa 23-474), a helical domain (aa 475-495) and a cytoplasmic domain (aa 496-571). Its cytoplasmic domain is shown to be essential for the apical membrane targeting and renal tubulogenesis. GP135 is involved in the regulation of both adhesion and cell morphology and cancer progression. It functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. It can also act as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands and thereby increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Gp135 is also reported to be involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. It co-localizes with actin filaments, ezrin and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Gp135 is shown to undergo N- and O-linked glycosylation.

Especificidad

Clone 3F2:D8 detects Podocalyxin in Madin-Darby canine kidney (MDCK) cells.

Inmunógeno

Epitope: extracellular domain

Intact Madin-Darby canine kidney (MDCK) cells.

Aplicación

Anti-Podocalyxin (GP135), clone 3F2:D8, Cat. No. MABS1327, is a mouse monoclonal antibody that detects Podocalyxin (GP135) and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Podocalyxin (GP135) in MDCK treated with iodoacetamid cell lysate.

Radioimmunoassay Analysis: A representative lot detected Podocalyxin (GP135) in Radioimmunoassay applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87).

Immunofluorescence Analysis: A representative lot detected Podocalyxin (GP135) in Immunofluorescence applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Podocalyxin (GP135) in MDCK cells (Courtesy of Dr. Tzuu-Shuh Jou, M.D., Ph.D., Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University).

Immunocytochemistry Analysis: A representative lot detected Podocalyxin (GP135) in Immunocytochemistry applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

Western Blotting Analysis: A representative lot detected Podocalyxin (GP135) in Western Blotting applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

Immunoprecipitation Analysis: A representative lot detected Podocalyxin (GP135) in Immunoprecipitation applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

Calidad

Evaluated by Western Blotting in MDCK cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Podocalyxin (GP135) in MDCK cell lysate.

Descripción de destino

180 kda obseerved; 59.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable