MABF946
Anti-H-2K Antibody, clone Y-3
clone Y-3, from mouse
Sinónimos:
H-2 class I histocompatibility antigen, K-B alpha chain, H-2K, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin
About This Item
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Y-3, monoclonal
species reactivity
mouse
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2bκ
NCBI accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
mouse ... H2-K1(14972)
General description
Specificity
Immunogen
Application
Affinity Binding Assay: A representative lot reacted with cell surface murine class I MHC H-2K haplotypes b, k, q, r, s, but not d, in affinity binding assays utilizing 125I-labelled antibody. Clone Y-3 exhibited similar affinity toward seven H-2Kb mutant strains as the wild-type strain (Hämmerling, G.J., et al. (1982). Proc. Natl. Acad. Sci. U. S. A. 79(15):4737-4741).
Flow Cytometry Analysis: A representative lot detected the surface expression of exogenously transfected H-2Kb Y84C mutant among TAP- and tapasin-deficient BL/6 fibroblasts, while little exogenously expressed wild-type H-2Kb was detected on the cell surface in the absence of either TAP or the TAP-binding protein tapasin (Wijeyesakere, S.J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(41):E5608-5617).
Flow Cytometry Analysis: Clone Y-3 ascites fluid detected an induciton of cell surface H-2K immunoreactivity upon exogenous murine calreticulin (mCRT) expression in the CRT-deficient K42 mouse fibroblast line (Del Cid, N., et al. (2010). J. Biol. Chem. 285(7):4520-4535).
Immunocytochemistry Analysis: A representative lot detected the surface expression of exogenously transfected H-2Kb Y84C mutant among TAP- and tapasin-deficient BL/6 fibroblasts, while exogenously expressed wild-type H-2Kb remained intracelluar in the absence of either TAP or the TAP-binding protein tapasin (Wijeyesakere, S.J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(41):E5608-5617).
Immunoprecipitation Analysis: A representative lot immunoprecipitated H2-K from a calreticulin-/CRT-deficient mouse embryonic fibroblast (MEF) line transfected to express murine CRT (Wijeyesakere, S.J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(41):E5608-5617).
Quality
Flow Cytometry Analysis: 0.1 µg of this antibody detected H-2K immunoreactivity on the surface of one million C57BL/6 mouse splenocytes (H-2 haplotype b).
Target description
Physical form
Other Notes
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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