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Key Documents

MABF599

Sigma-Aldrich

Anti-CD45.2 Antibody (mouse), PE-Cy7, clone 104

clone 104, 0.2 mg/mL, from mouse

Sinónimos:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

PE-Cy7

antibody form

purified antibody

antibody product type

primary antibodies

clone

104, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ptprc(19264)

General description

The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD45.2 (mouse), PE-Cy7, clone 104 validated for use in IP, Flow Cytometry & ICC for the detection of CD45.2.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg


Certificados de análisis (COA)

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