MABE328
Anti-MeCP2 Antibody, clone 4H7
clone 4H7, from rat
Sinónimos:
Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rat
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
4H7, monoclonal
reactividad de especies
mouse
reactividad de especies (predicha por homología)
rat (based on 100% sequence homology), porcine (based on 100% sequence homology)
técnicas
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG2aκ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
mouse ... Mecp2(17257)
Descripción general
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA. It represses transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
Especificidad
This antibody recognizes the C-terminus of MeCP2, which has been epitope mapped between amino acids 310-492.
Inmunógeno
Epitope: C-terminus
Full length, strep-tagged N-terminus of rat MeCP2.
Aplicación
Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse whole brain cell lysates (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This MeCP2 antibody is validated for use in WB, IHC, ICC, ChIP & IP for the detection of the MeCP2 protein
Calidad
Evaluated by Western Blot in mouse brain tissue nuclear extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract.
Descripción de destino
~75 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~55 kDa The calculated molecular weight is 53 kDa, however MeCP2 has been observed between ~75-100 kDa in western blots (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Forma física
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Nota de análisis
Control
Mouse brain tissue nuclear extract
Mouse brain tissue nuclear extract
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Melissa Mahgoub et al.
Nature neuroscience, 19(11), 1506-1512 (2016-10-28)
Class I histone deacetylases (HDACs) Hdac1 and Hdac2 can associate together in protein complexes with transcriptional factors such as methyl-CpG-binding protein 2 (MeCP2). Given their high degree of sequence identity, we examined whether Hdac1 and Hdac2 were functionally redundant in
Jessica Apulei et al.
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The non-cell autonomous transfer of OTX2 homeoprotein transcription factor into juvenile mouse cerebral cortex regulates parvalbumin interneuron maturation and critical period timing. By analyzing gene expression in primary visual cortex of wild-type and Otx2+/GFP mice at plastic and nonplastic ages
Camille Ravel-Godreuil et al.
iScience, 24(7), 102756-102756 (2021-07-20)
Age is a major risk factor for neurodegenerative diseases like Parkinson's disease, but few studies have explored the contribution of key hallmarks of aging, namely DNA methylation changes and heterochromatin destructuration, in the neurodegenerative process. Here, we investigated the consequences
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