MAB1959Z
Anti-Integrin beta 1 (CD29) Antibody
CHEMICON®, mouse monoclonal, P5D2
Sinónimos:
CD29, MAB1959
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Nombre del producto
Anti-Integrin β1 Antibody, clone P5D2 (Azide Free)| MAB1959, clone P5D2, Chemicon®, from mouse
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
P5D2, monoclonal
reactividad de especies
human
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotipo
IgG2bκ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ITGB1(3688)
Descripción general
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
Especificidad
Reacts with Human Integrin Beta 1.
Inmunógeno
Human keratinocytes derived from skin.
Aplicación
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA: A representative lot of this antibody was used in ELISA.
Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA: A representative lot of this antibody was used in ELISA.
Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Anti-Integrin β1 Antibody, clone P5D2 (Azide Free) detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IC, IH, IP, FUNC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Calidad
Flow Cytometry Analysis:
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
Forma física
Format: Purified
Protein A purified
Purified ascites in PBS without preservatives.
Almacenamiento y estabilidad
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
Nota de análisis
Control
Human tonsil, human skin tissue
A431 & HeLa Cells
Human tonsil, human skin tissue
A431 & HeLa Cells
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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