GR13L
Anti-EGFR (Ab-3) Mouse mAb (225)
lyophilized, clone 225, Calbiochem®
Sinónimos:
Anti-EGF-R
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
225, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
should not react with
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
Categorías relacionadas
General description
Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
Immunogen
Human
partially purified, human EGFR from A431 cells
Application
Immunoprecipitation (1-2 µg per sample)
Neutralization Studies (2 µg/ml, see comments and application references)
Neutralization Studies (2 µg/ml, see comments and application references)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
Reconstitution
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Analysis Note
Negative Control
HL-60 cells
HL-60 cells
Positive Control
A431 cells
A431 cells
Other Notes
Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Pallavi Garg et al.
American journal of physiology. Lung cellular and molecular physiology, 301(1), L79-L90 (2011-05-03)
Thrombospondin-1 (TSP1) is a multidomain protein that contains epidermal growth factor (EGF)-like repeats that indirectly activate the EGF receptor (EGFR) and selected downstream signaling pathways. In these studies, we show that TSP1 opens the paracellular pathway in human lung microvascular
Anguo Liu et al.
The Journal of biological chemistry, 284(10), 6389-6402 (2009-01-09)
Thrombospondin (TSP) 1 is a trimeric multidomain protein that contains motifs that recognize distinct host cell receptors coupled to multiple signaling pathways. Selected TSP1-induced cellular responses are tyrosine kinase-dependent, and TSP1 contains epidermal growth factor (EGF)-like repeats. Specific receptor interactions
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