ABT119

Anti-CELSR1 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Cadherin EGF LAG seven-pass G-type receptor 1, Cadherin family member 9, Flamingo homolog 2, hFmi2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), human (based on 100% sequence homology)
• técnicas: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_055061
• Nº de acceso UniProt: Q9NYQ6
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CELSR1(9620)

Descripción

Descripción general

The Flamingo/CELSR family of 7TM-cadherins are characterized with a symmetric protein distribution and polarized activity at neighboring epithelial cell interfaces along defined axes of planar cell polarity. As a component of the extracellular matrix, the CELSR1 protein has been found particularly at the basal surface of neuroepithelial cells within both the early neural tube and a less well-defined group of ventricular zone cells at the midline of the developing spinal cord. CELSR1 plays a role in orienting hair follicles along the anterior–posterior axis of the developing mouse epidermis, both during embryogenesis and postnatal development.

Inmunógeno

Linear peptide corresponding to human CELSR1.

Aplicación

Immunofluorescence Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected CELSR1 in rat cerebellum, substancia nigra, and hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected CELSR1 in neurons of rat cortex tissue and in Purkinje cells and cells within the granular layer of rat cerebellum tissue.

This Anti-CELSR1 Antibody is validated for use in Western Blotting, IHC, Immunofluorescence for the detection of CELSR1.

Calidad

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CELSR1 in 10 µg of PC12 cell lysate.

Descripción de destino

~330 kDa observed. A second isoform at ~152 kDa may be observed in some lysates.
An uncharacterized band at ~54 kDa may be observed in some cell lysates.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable