ABE1953
Anti-PTRF/Cavin-1 Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
Polymerase I and transcript release factor, PTRF/Cavin-1, Cavin-1
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
purificado por
affinity chromatography
reactividad de especies
rat, human
técnicas
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CAVIN1(284119)
Descripción general
Polymerase I and transcript release factor (UniProt Q6NZI2; also known as Cavin-1, PTRF, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12) is encoded by the PTRF (also known as CGL4, CAVIN, CAVIN1, FKSG13) gene (Gene ID 284119) in human. Cavin-1 is the founding member of the PTRF/Cavin family of cytoplasmic proteins whose expressions are essential for caveola formation. PTRF/Cavin-1, serum deprivation response (SDR)/Cavin-2, SDR-related gene product that binds to C kinase (SRBC)/Cavin-3, and muscle-restricted coiled-coil protein (MURC)/Cavin-4, form an oligomeric assembly termed Cavin complex in the cytosol and associate with caveolin at plasma membrane caveolae. Research shows an essential role for PTRF/Cavin-1 but not the other family members in caveola formation and recruitment of the cavin complex to the plasma membrane. In prostate cancer PC3 cells and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, where caveolin displays increased lateral mobility on the plasma membrane and accelerated lysosomal degradation. Expression of Cavin-1 restores caveolae formation in PC3 cells. Likewise the absence of cavin-1 is also observed in advanced prostate carcinoma. PTRF gene mutations are known to cause congenital generalized lipodystrophy 4 (CGL4).
Especificidad
Expected to react with spliced isoforms 1 and 2, but not isoform 3.
Inmunógeno
Linear peptide corresponding to the N-terminus of human PTRF/Cavin-1.
Aplicación
Anti-PTRF/Cavin-1 Antibody is an antibody against PTRF/Cavin-1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry.
Western Blotting Analysis: A representative lot detected PTRF/Cavin-1 expression in HeLa, but not in prostate cancer cell lines, LNCaP, 22Rv1 and PC-3 (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Western Blotting Analysis: A representative lot detected exogenously expressed human PTRF/Cavin-1 in transfected 22Rv1 and LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunohistochemistry Analysis: A representative lot detected reduced PTRF/Cavin-1 immunoreactivity in paraffin-embedded malignant prostate stroma tissue samples when compared with normal prostate stroma and benign prostatic hyperplasia (BPH) specimens. A lack of PTRF/cavin-1 immunoreactivity in prostate epithelia was seen among both normal and malignant samples (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunocytochemistry Analysis: A representative lot detected surface caveola localization of exogenously expressed human PTRF/Cavin-1 among transfected LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Western Blotting Analysis: A representative lot detected exogenously expressed human PTRF/Cavin-1 in transfected 22Rv1 and LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunohistochemistry Analysis: A representative lot detected reduced PTRF/Cavin-1 immunoreactivity in paraffin-embedded malignant prostate stroma tissue samples when compared with normal prostate stroma and benign prostatic hyperplasia (BPH) specimens. A lack of PTRF/cavin-1 immunoreactivity in prostate epithelia was seen among both normal and malignant samples (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunocytochemistry Analysis: A representative lot detected surface caveola localization of exogenously expressed human PTRF/Cavin-1 among transfected LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Calidad
Evaluated by Western Blotting in rat skeletal muscle myoblast L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PTRF/Cavin-1 in 10 µg of rat skeletal muscle myoblast L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PTRF/Cavin-1 in 10 µg of rat skeletal muscle myoblast L6 cell lysate.
Descripción de destino
~52 kDa observed.
Otras notas
Concentration: Please refer to lot specific datasheet.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Bayader Annabi et al.
International journal of stem cells, 10(1), 103-113 (2016-12-28)
Tumour necrosis factor (TNF)-α activation of mesenchymal stromal cells (MSC) enhances their tumour-suppressive properties and tumour-homing ability. The molecular actors involved are unknown. We found that TNF induced MSC migration and tubulogenesis which correlated with a dose-dependent increase in Cavin-1
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