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Key Documents

AB5735

Sigma-Aldrich

Anti-Neurofilament M Antibody

Chemicon®, from chicken

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

Specificity

Neurofilament M (NF-M)

Immunogen

Recombinant C-terminal extension of rat NF-M, the so-called KE segment.

Application

Anti-Neurofilament M Antibody detects level of Neurofilament M & has been published & validated for use in WB & IC.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Western blot: 1:5,000-1:10,000

Immunocytochemistry: 1:1,000-1:2,000

Optimal working dilutions must be determined by the end user.

Physical form

Chicken IgY. Liquid containing ~5 mM sodium azide.
Format: Purified

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Optional

Storage Class

10 - Combustible liquids


Certificados de análisis (COA)

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Karina Needham et al.
Stem cell research, 12(1), 241-249 (2013-11-28)
In severe cases of sensorineural hearing loss where the numbers of auditory neurons are significantly depleted, stem cell-derived neurons may provide a potential source of replacement cells. The success of such a therapy relies upon producing a population of functional
Niliksha Gunewardene et al.
Stem cells international, 2016, 1781202-1781202 (2016-03-12)
Induced pluripotent stem cells (iPSCs) may serve as an autologous source of replacement neurons in the injured cochlea, if they can be successfully differentiated and reconnected with residual elements in the damaged auditory system. Here, we explored the potential of
Rui O Costa et al.
Cell reports, 28(4), 864-876 (2019-07-25)
Ribosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes
Víctor Hugo Cornejo et al.
Cell reports, 30(13), 4505-4517 (2020-04-03)
TRPM8 is the main ion channel responsible for cold transduction in the somatosensory system. Nerve terminal availability of TRPM8 determines cold sensitivity, but how axonal secretory organelles control channel delivery remains poorly understood. Here we examine the distribution of TRPM8
Rodrigo López-Leal et al.
Methods in molecular biology (Clifton, N.J.), 1739, 255-267 (2018-03-17)
Sensory neurons from dorsal root ganglion efficiently regenerate after peripheral nerve injuries. These neurons are widely used as a model system to study degenerative mechanisms of the soma and axons, as well as regenerative axonal growth in the peripheral nervous

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