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AB1589P

Sigma-Aldrich

Anti-Adenosine A2b Receptor Antibody

Chemicon®, from rabbit

Sinónimos:

A2b Receptor Antibody, Adenosine A2b Antibody, Anti-Adenosine Receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ADORA2B(136)

Categorías relacionadas

Specificity

Adenosine A2b receptor. Recognizes a band of approximately 50-52 kDa in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). The antibody also recognizes the 35 kDa band in small intestine. No bands were detected in skeletal muscle or heart.

Immunogen

Amino acid sequence corresponding to the 2nd extracellular domain (16 amino acids) of the human brain A2bR gene (Pierce et al. 1992; Tucker & Linden 1993).

Application

Detect Adenosine A2b Receptor using this Anti-Adenosine A2b Receptor Antibody validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen and formalin fixed tissue sections. The receptor was localized in epithelial cells of the crypts, and in synctiotrophoplast cells of human placenta and in T84 human colon adenocarcinoma cells.

Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Circadian Rhythm & Sleep

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Shefeeq M Theparambil et al.
Nature, 632(8023), 139-146 (2024-07-04)
Brain computation performed by billions of nerve cells relies on a sufficient and uninterrupted nutrient and oxygen supply1,2. Astrocytes, the ubiquitous glial neighbours of neurons, govern brain glucose uptake and metabolism3,4, but the exact mechanisms of metabolic coupling between neurons
Elizabeth Salvo et al.
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Pain associated with oral squamous cell carcinoma (oral SCC) decreases quality of life and survival. The interaction between cancer and the peripheral nerves is known to initiate and amplify pain and contribute to carcinogenesis. Schwann cells envelop peripheral nerves and
Zuokang Zheng et al.
Cell death & disease, 9(11), 1133-1133 (2018-11-16)
MicroRNAs are a group of small non-coding RNAs that play key roles in almost every aspect of mammalian cell. In kidney, microRNAs are required for maintaining normal function of renal cells, disruption of which contributes to pathogenesis of renal diseases.
Alejandro Sánchez-Melgar et al.
Nutrients, 13(9) (2021-09-29)
Neurodegenerative disorders are devastating diseases in which aging is a major risk factor. High-fat diet (HFD) seems to contribute to cognition deterioration, but the underlying mechanisms are poorly understood. Moreover, resveratrol (RSV) has been reported to counteract the loss of
Tadeusz Pawelczyk et al.
The American journal of pathology, 167(2), 315-325 (2005-07-29)
Pathological alterations of renal function in insulin-dependent diabetes have been attributed to numerous factors, including adenosine. This study examined the expression levels of adenosine receptors (ARs) in the kidney of the streptozotocin-induced diabetic rat. In the diabetic kidney A1-AR mRNA

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