33-017
PI 3-Kinase HTRF® Assay; 1920 wells
The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.
Sinónimos:
Kinase activity assay
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Productos recomendados
Nivel de calidad
reactividad de especies
mouse, human
fabricante / nombre comercial
HTRF®
Upstate®
técnicas
activity assay: suitable (kinase)
Nº de acceso NCBI
Nº de acceso UniProt
método de detección
fluorometric (HTRF)
Condiciones de envío
dry ice
Información sobre el gen
human ... PIK3CA(5290)
Descripción general
The PI3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for assaying all Class I PI3-Kinases in a homogeneous 384-well format.
Aplicación
PI 3-Kinase HTRF® Assay; 1920 wells have been used to measure the activity of phosphoinositide-3-kinases (PI3Ks). It has also been used in a biochemical assay to measure the potency and selectivity of LL-00071210 (a selective PI3Kδ inhibitor).
Acciones bioquímicas o fisiológicas
The PI 3-Kinases catalyze the phosphorylation of the three positions of phosphatidylinositols. The Class I enzymes convert phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg2+.
Características y beneficios
- Large Stokes shift and time-resolved elements of technology help to reduce compound interference, minimizing concern of false negatives
- Picomole sensitivity of the HTRF assay platform enables the integrity of enzyme kinetics to be retained, ensuring the most physiologically relevant assay parameters.
- Compatible with all Class I phosphoinositide 3-kinase (PI3K) isoforms: enables relative data comparisons and eliminates the need for multiple assay types.
- Reliable, proven assay—based on the technology used in the Millipore, Upstate® KinaseProfiler Service.
- Z` factor routinely exceeds 0.8, ensuring consistent, reliable data.
Envase
Kit capacity: 1920 wells
Componentes
4X Reaction Buffer
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
Información legal
HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Reine Nehmé et al.
Analytical and bioanalytical chemistry, 406(15), 3743-3754 (2014-05-13)
Human kinases are one of the most promising targets for cancer therapy. Methods able to measure the effects of drugs on these cell agents remain crucial for biologists and medicinal chemists. The current work therefore sought to develop an in-capillary
Robert H Sinnamon et al.
Protein expression and purification, 73(2), 167-176 (2010-05-12)
Phosphoinositide 3-kinases have been targeted for therapeutic research because they are key components of a cell signaling cascade controlling proliferation, growth, and survival. Direct activation of the PI3Kalpha pathway contributes to the development and progression of solid tumors in breast
Marinela Méndez-Pertuz et al.
Nature communications, 8(1), 1278-1278 (2017-11-04)
Telomeres and the insulin/PI3K pathway are considered hallmarks of aging and cancer. Here, we describe a role for PI3K/AKT in the regulation of TRF1, an essential component of the shelterin complex. PI3K and AKT chemical inhibitors reduce TRF1 telomeric foci
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