09-272

Anti-phospho-p38α (Thr180/Tyr182) Antibody

Upstate^®, from rabbit

• Sinónimos: SAPK2a, MAPK14, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, p38ALPHA, mitogen-activated protein kinase 14
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, rat, human
• envase: antibody small pack of 25 μL
• fabricante / nombre comercial: Upstate^®
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_001315.1; NM_139012.1; NM_139013.1; NM_139014.1
• Nº de acceso UniProt: Q16539
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: phosphorylation (pThr180/pTyr182)
• Información sobre el gen: human ... MAPK14(1432)

Descripción

Especificidad

Recognizes p38α dual phosphorylation of Thr180 and Tyr182.

Inmunógeno

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).

Aplicación

Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.

Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.

Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.

Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).

Calidad

Routinely evaluated by immunoblot.

Descripción de destino

38 kDa

Ligadura / enlace

Replaces: AB3828

Forma física

ImmunoAffinity Purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Almacenamiento y estabilidad

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Anisomycin treated HeLa cells

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.