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Key Documents

07-789

Sigma-Aldrich

Anti-phospho-AKT (Thr34) Antibody

Upstate®, from rabbit

Sinónimos:

Akt1, PKB, PRKBA, RAC, RAC-ALPHA

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr34)

Gene Information

human ... AKT1(207)

Specificity

Predicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
Recognizes phospho-AKT (Thr34)

Immunogen

KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.

Application

Anti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

General Post-translation Modification

Quality

Routinely evaluated by immunoblot.

Target description

60 kDa

Physical form

100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Storage and Stability

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
Marte, B M and Downward, J
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