07-1538

Anti-JMJD4 (NT) Antibody

from rabbit

• Sinónimos: JMJD, Jumonji domain-containing protein 4, jumonji domain containing 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• technique(s): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_075383.1
• UniProt accession no.: Q9H9V9
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... JMJD4(65094)

Descripción

General description

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that remove specific methyl marks from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues

Specificity

This antibody recognizes the N-terminal region of human JMJD4.

Immunogen

Epitope: N-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD4.

Application

ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA.

Immunocytochemistry:
HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Chromatin Biology

Use Anti-JMJD4 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD4 (N-terminus) also known as Jumonji domain-containing protein 4.

Quality

Western Blot Analysis:
1:250 dilution of this antibody detected JMJD4 on 10 µg of A431 lysate

Target description

~53 kDa

Physical form

Format: Purified

Protein G Chromatography

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage and Stability

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
A431 lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable