05-613
Anti-β-Catenin Antibody, clone 2H4A7
clone 2H4A7, Upstate®, from mouse
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2H4A7, monoclonal
Application:
IHC, IP, WB
Citations:
9
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2H4A7, monoclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ctnnb1(12387)
General description
92kDa
Immunogen
fusion protein corresponding to residues 1-100 of human β-Catenin
Application
Detect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 2H4A7 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP, WB & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Biochem/physiol Actions
β-Catenin
Physical form
Format: Purified
Protein G Chromatography
Protein G purified IgG in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
Preparation Note
Stable 2 years at 2-8°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from A431, mouse 3T3/A31 or rat L6 cell lysates
Other Notes
Replaces: 04-1002
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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