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901756

Sigma-Aldrich

Low endotoxin gelatin from porcine skin

gel strength 240-360 (Bloom), <10 EU/g Endotoxin

Sinónimos:

beMatrix™ gelatin LS-H

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About This Item

Número de CAS:
9000-70-8
Número MDL:
MFCD00564673
Código UNSPSC:
41116130
NACRES:
NA.23

Formulario

powder

impurezas

<10 EU/g Endotoxin
<300 g Total viable aerobic count

pérdida

<15% loss on drying

color

white to pale yellow

pH

5.0-6.5

viscosidad

4.0-6.5 mPa.s

trazas de anión

sulfite (SO32-): <1.5 mg/20g

trazas de catión

As: <1 ppm
Hg: <0.1 ppm
heavy metals: <20 ppm

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Aplicación

Gelatin has received significant attention in the biomedical field due to its inherent bioactivity. Low endotoxin gelatin from porcine skin, gel strength 300 bloom is a high bloom (high molecular weight) gelatin featuring less than 10 endotoxin units (EU) per gram. Endotoxins primarily consist of lipopolysaccharides from the outer membrane of gram-negative bacteria. Once in the bloodstream, endotoxins are capable of eliciting a strong immune response, potentially leading to fever, shock, a drop in blood pressure, and even potentially death. In addition to this immune response, several reports suggest endotoxins may also damage various types of cells. To avoid adverse effects, raw materials should contain the lowest level of endotoxins possible when used in biomedical or regenerative medicine research applications. This low endotoxin gelatin is derived from alkaline-treated porcine skin and has a variety of potential applications in the fields of regenerative medicine, medical devices, and tissue engineering research.

Información legal

beMatrix is a trademark of Nitta Gelatin

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
MKCQ8667

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K W Brunson et al.
Journal of supramolecular structure, 9(2), 231-242 (1978-01-01)
Chinese hamster ovary (CHO . K1 . PRO) cell growth was inhibited by addition of a gram-negative bacterial lipopolysaccharide (LPS) to the cell culture medium. Growth inhibition began after three or four days of incubation, was dose-dependent up to a
J M Harlan et al.
Laboratory investigation; a journal of technical methods and pathology, 48(3), 269-274 (1983-03-01)
Lipopolysaccharide (LPS) produced time- and dose-dependent bovine endothelial cell injury in vitro that was manifested initially by cell detachment from culture substrate with subsequent cell lysis. Bovine endothelial cell injury was observed with LPS derived from Salmonella minnesota R595, a
J G Brock-Utne et al.
Anaesthesia and intensive care, 17(1), 49-55 (1989-02-01)
Endotoxins (lipopolysaccharides, LPS) are potent bacterial poisons always present within the intestines in considerable amounts. Several pathophysiological conditions such as hypovolaemia, hypoxia, intestinal ischaemia, burns and radiation lead to a breakdown in the barrier and depending upon the extent of
E J Ziegler et al.
The New England journal of medicine, 307(20), 1225-1230 (1982-11-11)
In an effort to decrease deaths from gram-negative bacteremia and endotoxin shock, we treated bacteremic patients with human antiserum to endotoxin (lipopolysaccharide) core. Antiserum was prepared by vaccinating healthy men with heat-killed Escherichia coli J5; this mutant lacks lipopolysaccharide oligosaccharide
T Kirikae et al.
International journal of immunopharmacology, 19(5), 255-262 (1997-05-01)
Trace amounts of endotoxin (lipopolysaccharide: LPS) are assumed to contaminate commercially available fetal bovine serum (FBS) for tissue or cell culture during the manufacturing process. We examined how cultured cells were affected by the endotoxin and how much endotoxin was
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