T1895

Thymidine

powder, BioReagent, suitable for cell culture

• Synonym(s): 1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂O₅
• CAS Number: 50-89-5
• Molecular Weight: 242.23
• Beilstein: 89285
• EC Number: 200-070-4
• MDL number: MFCD00006537
• UNSPSC Code: 12352207
• PubChem Substance ID: 24899996
• NACRES: NA.75

Properties

• biological source: synthetic (organic)
• product line: BioReagent
• Assay: ≥99%
• form: powder
• technique(s): cell culture | mammalian: suitable
• mp: 186-188 °C (lit.)
• solubility: water: 50 mg/mL, clear, colorless
• SMILES string: CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
• InChI: 1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
• InChI key: IQFYYKKMVGJFEH-XLPZGREQSA-N
• Gene Information: human ... DTYMK(1841)

Description

General description

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.

Application

Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:

• as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
• as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
• to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells

Biochem/physiol Actions

Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves