S0692

Spectinomycin

Ready Made Solution, 100 mg/mL in DMSO/H₂O, 1:1

• Synonym(s): Actinospectacin
• MDL number: MFCD04041129
• UNSPSC Code: 51281602
• NACRES: NA.85

Properties

• biological source: Streptomyces sp.
• Quality Level: 200
• form: solution
• concentration: 100 mg/mL in DMSO/H₂O, 1:1
• color: colorless
• solubility: DMSO/H₂O, 1:1: soluble 100 mg/mL
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• Mode of action: protein synthesis | interferes
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Chemical structure: aminoglycoside

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.

Storage and Stability

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 201.0 °F - closed cup
• Flash Point(C): 93.9 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves