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RAB0715

Sigma-Aldrich

Human MB / Myoglobin ELISA Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.82 ng/mL
standard curve range: 0.819-200 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MB(4151)

Related Categories

General description

The MB ELISA kit provides for the Quantitative measurement of Myoglobin in Cell Culture Supernatants, Plasma and Serum.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Myoglobin is a hemoprotein that regulates the storage and diffusion of oxygen in heart and skeletal muscles. It also protects the tissues from oxidative damage by controlling the levels of reactive oxygen species and nitric oxide. Myoglobin modulates nitric oxide and oxygen levels in the mitochondrial compartments of skeletal muscle and cardiac cells.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Myoglobin: an essential hemoprotein in striated muscle.
Ordway GA, et al.
The Journal of Experimental Biology, 207(20), 3441-3446 (2004)
Xiaoyun Liu et al.
International journal of cardiology, 296, 129-135 (2019-08-24)
The therapeutic potential of doxorubicin (DOX) is limited by cardiotoxicity. Rubicon is an inhibitory interacting partner of autophagy protein UVRAG. Currently, the role of Rubicon in DOX-induced cardiotoxicity is unknown. In this study, we test the hypothesis that loss of
Fangfei Li et al.
Nature communications, 8(1), 1390-1390 (2017-11-11)
Paclitaxel (PTX) is among the most commonly used first-line drugs for cancer chemotherapy. However, its poor water solubility and indiscriminate distribution in normal tissues remain clinical challenges. Here we design and synthesize a highly water-soluble nucleolin aptamer-paclitaxel conjugate (NucA-PTX) that
Lin An et al.
Scientific reports, 7, 43251-43251 (2017-02-23)
Doxorubicin (DOX) is an effective chemotherapeutic drug in the treatment of various types of cancers. However, its clinical application has been largely limited by potential development of cardiotoxicity. Previously we have shown that ultra-violet radiation resistance-associated gene (UVRAG), an autophagy-related
Meng Jiang et al.
Clinical laboratory, 64(7), 1207-1212 (2018-08-28)
This study aims to evaluate the expression of plasma miR-18, thereby evaluating its potential use as a biomarker to screen acute myocardial infarction (AMI) patients from healthy controls. Real time PCR was carried out to evaluate the level of miR-18
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