P0091
PolyFreeze
water-soluble support matrix
Synonym(s):
water-soluble support matrix for frozen sectioning
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.47
Recommended Products
color
colorless
density
1.030 g/cm3
application(s)
hematology
histology
storage temp.
room temp
General description
PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen, or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer. PolyFreeze does not cause autofluorescence and can easily be washed away during fixation and rinsed prior to staining. Once rinsed, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.
Application
PolyFreeze has been used as an embedding medium for frozen sectioning of plant and animal samples.
Preparation Note
PolyFreeze is supplied ready-to-use. A cloudy appearance of the unfrozen product has no effect on its performance.
Storage and Stability
Store the product at room temperature
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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