M5409
Mouse IgG2a Isotype Control from murine myeloma
clone UPC-10, purified immunoglobulin, buffered aqueous solution
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
UPC-10, monoclonal
form
buffered aqueous solution
technique(s)
flow cytometry: suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
Specificity
When evaluated in flow cytometry, the product did not stain human peripheral blood lymphocytes (PBLs). Anti-Mouse IgG (whole molecule), F(ab′)2 fragment FITC, Catalog Number F2883, along with 1 mg of the product was incubated with human PBLs and then evaluated by flow cytometry.
Application
Mouse IgG2a Isotype Control from murine myeloma has been used in:
- immunoprecipitation
- enzyme-linked immunosorbent assay (ELISA)
- fluorescence microscopy
- indirect immunocytochemistry
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Correlative microscopy of lamellar hole-associated epiretinal proliferation
Compera D, et al.
Journal of Ophthalmology, 2015, 1829-1837 (2015)
Denise Vogt et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 256(9), 1589-1597 (2018-06-23)
To investigate integrity and characteristics of human premacular membranes (PMM) with and without standard tissue culturing using mechanical traction. Premacular membranes were harvested from 32 eyes of 32 patients with idiopathic macular pucker during standard vitrectomy. By flat-mount preparation with
Chantal Beekman et al.
PloS one, 9(9), e107494-e107494 (2014-09-23)
Duchenne muscular dystrophy (DMD) is characterized by the absence or reduced levels of dystrophin expression on the inner surface of the sarcolemmal membrane of muscle fibers. Clinical development of therapeutic approaches aiming to increase dystrophin levels requires sensitive and reproducible
Activation of the p53 pathway induces alpha-smooth muscle actin expression in both myeloid leukemic cells and normal macrophages
Secchiero P, et al.
Journal of Cellular Physiology, 227(5), 1829-1837 (2012)
Cellular stress induced alterations in microRNA let-7a and let-7b expression are dependent on p53
Saleh AD, et al.
Testing, 6(10), e24429-e24429 (2011)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service