H9950
Hanahan′s Broth (SOB Medium)
Liquid microbial growth medium
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About This Item
Recommended Products
grade
for molecular biology
sterility
sterile; 0.2 μm filtered
form
liquid
suitability
suitable for nonselective for Escherichia coli and Coliforms
storage temp.
room temp
General description
Hanahan′s (SOB) media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
Hanahan′s (SOB) media provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Components
20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4
Physical form
20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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