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F7378

Sigma-Aldrich

Fluorescein diacetate

Powder

Synonym(s):

3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
596-09-8
Molecular Weight:
416.38
Beilstein:
62575
EC Number:
209-877-6
MDL number:
MFCD00005062
UNSPSC Code:
12171500
PubChem Substance ID:
24894939
NACRES:
NA.47

product name

Fluorescein diacetate, used as cell viability stain

form

powder

color

white to faint yellow

mp

200-203 °C (lit.)

solubility

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

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General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Substrates

Lipase substrate

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhang J
Biomedical Materials (Bristol, England), 9, 035011-035011 (2014)
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Effect of shock waves on suspended and immobilized L1210 cells.
Brummer F
Ultrasound in Medicine & Biology, 15, 229-229 (1989)
Melina M Malinen et al.
Tissue engineering. Part A, 18(23-24), 2418-2425 (2012-06-21)
Current hepatocyte models do not mimic the human liver morphology and functions properly and, therefore, drug metabolism, excretion, and toxicity in the liver are inadequately predicted. In this study, we established three-dimensional (3D) hepatic cell cultures in hydrogels of peptide
Derek Toms et al.
Frontiers in bioengineering and biotechnology, 8, 534-534 (2020-06-26)
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