MDCK

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011435, dog kidney, Epithelial-like

• Synonym(s): MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32190102

Properties

• biological source: dog kidney
• packaging: tube of 5 μg 85011435-DNA-5UG; pkg of vial of cells 85011435-1VL
• growth mode: Adherent
• karyotype: 2n = 78
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Canine Cocker Spaniel kidney

Cell Line Description

Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

Application

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.