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E9379

Sigma-Aldrich

Stains-All

~95% purity, powder

Synonym(s):

1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine

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About This Item

Empirical Formula (Hill Notation):
C30H27BrN2S2
CAS Number:
Molecular Weight:
559.58
Beilstein:
3865213
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Stains-All, ~95%

Assay

~95%

Quality Level

form

powder

solubility

chloroform: 40 mg (PLUS 4.0 ml)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC

InChI

1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

MPBRYMWMMKKRGC-UHFFFAOYSA-M

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Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Biochem/physiol Actions

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Caution

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Chromatin and Chromosomal Protein Research III (1978)
Kinetics of duplex formation for individual DNA strands within a single protein nanopore.
Howorka S, et al.
Proceedings of the National Academy of Sciences of the USA, 98, 12996-12996 (2001)
Rehm H
Protein Biochemistry and Proteomics. The experimenter series (2006)
A comparison of the sensitivity, specificity, and molecular weight accuracy of three different commercially available Hyaluronan ELISA-like assays.
Haserodt S, et al.
Glycobiology, 21, 175-175 (2011)
Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.
Lauer ME, et al.
The Journal of Biological Chemistry, 284, 5299-5299 (2009)

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