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10843555001

Roche

Hygromycin B

from Streptomyces hygroscopicus

Synonym(s):

Hygromycin B from Streptomyces hygroscopicus

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About This Item

Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
31282-04-9
Molecular Weight:
527.52
MDL number:
MFCD06795479
UNSPSC Code:
12352200
PubChem Substance ID:
329749191

biological source

Streptomyces hygroscopicus

sterility

non-sterile; 0.2 μm filtered

Assay

80% (HPLC)

form

buffered aqueous solution

composition

Hygromycin B, >80% HPLC

packaging

pkg of 20 mL (1 g)

manufacturer/tradename

Roche

concentration

50 mg/mL

technique(s)

transfection: suitable

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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General description

  • Formula: C20H37N3O13
  • LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
  • Molecular weight: Mr = 527.5
  • Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Application

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Quality

Purity: >80% (HPLC)

Unit Definition

Unit Assay: Unit/mg
We do not have this information.

Physical form

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

Storage and Stability

Store at 2 to 8 °C. (solution)

Other Notes

For life science research only. Not for use in diagnostic procedures.
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nucleic acids research, 41(16), e158-e158 (2013-07-19)
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Charlotte Vandermeulen et al.
PLoS pathogens, 17(9), e1009919-e1009919 (2021-09-21)
Viral infections are known to hijack the transcription and translation of the host cell. However, the extent to which viral proteins coordinate these perturbations remains unclear. Here we used a model system, the human T-cell leukemia virus type 1 (HTLV-1)
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Cell reports, 33(3), 108287-108287 (2020-10-22)
Homologous recombination is initiated by nucleolytic degradation (resection) of DNA double-strand breaks (DSBs). DSB resection is a two-step process in which an initial short-range step is catalyzed by the Mre11-Rad50-Xrs2 (MRX) complex and limited to the vicinity of the DSB
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