OP44

Anti-APC (Ab-1) Mouse mAb (FE9)

liquid, clone FE9, Calbiochem^®

• Synonym(s): Anti-Adenomatous Polyposis Coli
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: FE9, monoclonal
• Species reactivity: rat, human, mouse
• Application: —
• Citations: 23

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: FE9, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Immunoblotting (1 µg/mL))
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... APC(324)

Description

General description

Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.

• Antibody Target Gene Symbol: APC
• Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
• Entrez Gene Name: adenomatous polyposis coli
• Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L)
• Mu Entrez ID: 11789
• Rat Entrez ID: 24205

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.

Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.

Immunogen

a synthetic peptide corresponding to the N-terminal 35 amino acids of APC

Application

Immunoblotting (1 µg/ml, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.

Analysis Note

Positive Control
HCT116 cells for p300, SW480 cells for truncated APC (p147)

Other Notes

Koetsier, P. A., et al. 1993. BioTechniques15, 258.
Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
Su, L.-K., et al. 1993. Can. Res.53, 2728.
Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
D′Amico, D., et al. 1992. Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
Powell, S. M., et al. 1992. Nature359, 235.
Groden, J., et al. 1991. Cell66, 589.
Kinzler, K. W., et al. 1991. Science253, 661.
Nishisho, I., et al. 1991. Science253, 665.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable