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MABF121

Sigma-Aldrich

Anti-SV40 Large T Antigen Antibody, clone PAb416

clone PAb416, from mouse

Synonym(s):

Large T antigen, LT, LT-AG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

PAb416, monoclonal

species reactivity

SV40-infected cells

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

Specificity

This antibody recognizes the large T antigen of SV40.

Immunogen

Epitope: Large T antigen of SV40
Purified large T-antigen of SV40

Application

Anti-SV40 Large T Antigen Antibody, clone PAb416 is an antibody against SV40 Large T Antigen for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SV40 Large T Antigen in ICC (Sabatier, J., et al. (2005). 58(4):429-431.; Del Valle, L., et al. (2004). J Virol. 78(7):3462-3469.).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blot in Cos-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SV40 Large T Antigen in 10 µg of Cos-1 cell lysate.

Target description

~82 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Cos-1 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ursula Neu et al.
PLoS pathogens, 9(10), e1003688-e1003688 (2013-10-17)
Viruses within a family often vary in their cellular tropism and pathogenicity. In many cases, these variations are due to viruses switching their specificity from one cell surface receptor to another. The structural requirements that underlie such receptor switching are
Yasuko Orba et al.
The Journal of general virology, 92(Pt 4), 789-795 (2010-12-24)
To investigate polyomavirus infection in wild rodents, we analysed DNA samples from the spleens of 100 wild rodents from Zambia using a broad-spectrum PCR-based assay. A previously unknown polyomavirus genome was identified in a sample from a multimammate mouse (Mastomys
Furong Yuan et al.
Oncology reports, 23(2), 377-386 (2010-01-01)
Werner syndrome (WS) results from defects in the gene encoding WRN RecQ helicase. WS fibroblasts undergo premature senescence in culture. Because cellular senescence is a tumor suppressor mechanism, we examined whether WS fibroblasts exhibited reduced tumorigenicity, in comparison to control
Linda C Knight et al.
Journal of molecular biomarkers & diagnosis, 1(1) (2010-01-01)
A targeted nanoconjugate is being developed for non-invasive detection of gene expression in cells expressing the JC virus oncoprotein, T-antigen, which has been associated with medulloblastoma and other cancers. JC virus T-antigen localizes predominantly to the nucleus via a classical
Sonia Ramamoorthy et al.
Cancer, 117(11), 2379-2385 (2011-06-01)
Anal carcinoma is thought to be driven by human papillomavirus (HPV) infection through interrupting function of cell regulatory proteins such as p53 and pRb. John Cunningham virus (JCV) expresses a T-antigen that causes malignant transformation through development of aneuploidy and

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