MAB308
Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2
ascites fluid, clone 4F10.2, Chemicon®
Synonym(s):
DBH
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
4F10.2, monoclonal
species reactivity
rat, bovine, sheep
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Dbh(280758)
rat ... Dbh(25699)
General description
Dopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.
Specificity
Dopamine beta hydroxylase (DBH).
Immunogen
Purified bovine DBH.
Application
Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.
Indirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
1:300-1:500 dilution of a previous lot was used on rat brain tissue.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Quality
Immunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Linkage
Replaces: MAB394-250UG; MAB394-100UG; MAB394-25UG
Physical form
Ascites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Differential activation of chemically identified neurons in the caudal nucleus of the solitary tract in non-entrained rats after intake of satiating vs. non-satiating meals.
Kreisler, AD; Davis, EA; Rinaman, L
Physiology & Behavior null
The rate of fall of blood glucose determines the necessity of forebrain-projecting catecholaminergic neurons for male rat sympathoadrenal responses.
Jokiaho, AJ; Donovan, CM; Watts, AG
Diabetes null
Glucocorticoid enhancement of memory requires arousal-induced noradrenergic activation in the basolateral amygdala.
Roozendaal, B; Okuda, S; Van der Zee, EA; McGaugh, JL
Proceedings of the National Academy of Sciences of the USA null
Michael D Schwartz et al.
eNeuro, 3(2) (2016-03-30)
Hypocretin 1 and 2 (Hcrts; also known as orexin A and B), excitatory neuropeptides synthesized in cells located in the tuberal hypothalamus, play a central role in the control of arousal. Hcrt inputs to the locus coeruleus norepinephrine (LC NE)
S Gutierrez et al.
Neuroscience, 228, 301-308 (2012-10-30)
Tissue and nerve damage can result in chronic pain. Yet, chronic pain after cesarean delivery is remarkably rare in women and hypersensitivity from peripheral nerve injury in rats resolves rapidly if the injury occurs in the puerperium. Little is known
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service