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MAB3034

Sigma-Aldrich

Anti-DNA Antibody, single stranded, clone 16-19

clone 16-19, Chemicon®, from mouse

Synonym(s):

Single-Stranded DNA Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

16-19, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable

isotype

IgG2aκ

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.

ANTIGEN BINDING PROFILE:

Relative Binding To:

Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53

a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA

Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).

BINDING ASSAY

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.

ISOELECTRIC POINT = 8.3

Immunogen

Epitope: single stranded DNA

Application

ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.
Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Cell Cycle, DNA Replication & Repair

Physical form

0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cyclin-dependent kinase regulates the length of S phase through TICRR/TRESLIN phosphorylation.
Sansam, CG; Goins, D; Siefert, JC; Clowdus, EA; Sansam, CL
Genes & Development null
Genome instability and transcription elongation impairment in human cells depleted of THO/TREX.
Dominguez-Sanchez, MS; Barroso, S; Gomez-Gonzalez, B; Luna, R; Aguilera, A
PLoS Genetics null
Anne Fernandez-Vidal et al.
Nature communications, 5, 4285-4285 (2014-07-06)
Although DNA polymerase θ (Pol θ) is known to carry out translesion synthesis and has been implicated in DNA repair, its physiological function under normal growth conditions remains unclear. Here we present evidence that Pol θ plays a role in
Therese Wilhelm et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(2), 763-768 (2013-12-19)
Homologous recombination deficient (HR(-)) mammalian cells spontaneously display reduced replication fork (RF) movement and mitotic extra centrosomes. We show here that these cells present a complex mitotic phenotype, including prolonged metaphase arrest, anaphase bridges, and multipolar segregations. We then asked
Alberto Ciccia et al.
Genes & development, 23(20), 2415-2425 (2009-10-02)
The integrity of genomic DNA is continuously challenged by the presence of DNA base lesions or DNA strand breaks. Here we report the identification of a new DNA damage response protein, SMARCAL1 (SWI/SNF-related, matrix associated, actin-dependent regulator of chromatin, subfamily

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