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AB1837P

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody

Chemicon®, from rabbit

Synonym(s):

TNF-alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

Specificity

Reacts with rat TNF-alpha

Immunogen

Highly purified (>98%) recombinant rat TNF-alpha

Application

Anti-Tumor Necrosis Factor-α Antibody is an antibody against Tumor Necrosis Factor-α for use in ELISA & WB.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of

0.2-0.4 ng/well when rat TNF-α is used to coat the plate.

Western blot: 0.1 - 0.2 μg/mL, this dilution should offer detection limit of 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.

Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Linkage

Replaces: 04-1114

Physical form

Affinity Purified Immunoglobulin. Lyophilized from PBS pH 7.2. Contains no preservative. Reconstitute with sterile distilled water.

Storage and Stability

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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X J Fei et al.
Genetics and molecular research : GMR, 13(4), 10537-10544 (2014-12-17)
The dried roots of the plant Acanthopanax senticosus (AS) are used in traditional Oriental medicine and reportedly possess anti-inflammatory properties in vitro. However, whether AS has the same anti-inflammatory effect in vivo and the underlying mechanisms of this action remain
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Rejuvenation research, 13(1), 65-74 (2010-03-17)
Aging is associated with protein damage and imbalance in redox status in a variety of cells and tissues, yet little is known about the extent of age-related oxidative stress in the peripheral nervous system. Previously, we showed a drastic decline
Fan Li et al.
Journal of neurochemistry, 106(5), 2093-2105 (2008-07-17)
Microglial cells are endowed with different potassium ion channels but their expression and specific functions have remained to be fully clarified. This study has shown Kv1.2 expression in the amoeboid microglia in the rat brain between 1 (P1) and 10
Clémence Guiraut et al.
International journal of molecular sciences, 17(12) (2016-11-30)
The medium-size intra-cranial arteries arising from the carotid bifurcation are prone to perinatal arterial ischemic strokes (PAIS). PAIS' physiopathology needs to be better understood to develop preventive and therapeutic interventions that are currently missing. We hypothesized that materno-fetal inflammation leads
Talal Al-Shatti et al.
Journal of neuroimmunology, 167(1-2), 13-22 (2005-07-20)
We examined cytokines in rat median nerves following performance of a high repetition reaching and grasping task at a rate of 8 reaches/min for up to 8 weeks. IL-1alpha, IL-1beta, TNF-alpha, IL-6 and IL-10 were analyzed by immunohistochemistry. Double-labeling immunohistochemistry

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