The isolation/extraction method for Rat Genomic DNA is proprietary and unavailable. The DNA is obtained from multiple animals from a single strain (Sprague Dawley) and a mixed sex population. The isolation protocol minimizes shearing and removes contaminants that inhibit enzymatic procedures. The DNA's origin, whether a mixture of mitochondrial and nuclear, solely mitochondrial, or nuclear, is not evaluated as part of the quality control process.
69238
Rat Genomic DNA
High molecular weight, high purity DNA for any application
Synonym(s):
Rat DNA, Rat genomic DNA
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About This Item
NACRES:
NA.51
UNSPSC Code:
12352208
Form:
liquid
Solubility:
water: soluble at 20 °C
Storage temp.:
2-8°C
Pricing and availability is not currently available.
Quality Segment
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
solubility
water: soluble at 20 °C
shipped in
wet ice
storage temp.
2-8°C
General description
Novagen genomic DNA is a purified preparation of high molecular weight genomic DNA obtained from various organisms. During the isolation process, measures are taken to minimize shearing and eliminate contaminants that could disrupt enzymatic procedures. It has been qualified for genomic analysis, including library construction and PCR, as at least 90% of the DNA is greater than 100 kbp in size, with contour-clamped homogeneous electric field (CHEF) electrophoresis analysis showing sizes greater than 50 kbp for S. cerevisiae. Notably, no inhibition of restriction enzyme activity has been observed, and the DNA is free from protein and RNA contaminants.
Application
Ratgenomic DNA has been used as a template for the amplification of theprogression elevated gene-3 (PEG-3) gene using polymerase chain reaction (PCR).
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
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| description High molecular weight, high purity DNA for any application | description High molecular weight, high purity DNA for any application | description High molecular weight, high purity DNA for any application | description from human blood (buffy coat) |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level - |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Roche |
| form liquid | form liquid | form - | form solution |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| solubility water: soluble at 20 °C | solubility water: soluble at 20 °C | solubility - | solubility water: miscible |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
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Regarding cat no. 69238 Rat Genomic DNA, which extraction method was used to extract the HMW DNA? We are specifically interested in rat mitochondrial DNA, is this still included?
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