07-463

Anti-trimethyl-Histone H4 (Lys20) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Histone H4 (trimethyl Lys20), H4K20me3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, mouse, rat
• Application: ChIP, DB, IP, WB
• Citations: 119

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• technique(s): ChIP: suitable, dot blot: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_001029249
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: trimethylation (Lys20)
• Gene Information: human ... H4C1(8359) ; mouse ... H4C1(326619)

Description

General description

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.

Immunogen

Epitope: Trimethylated Lys20

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.

Application

Anti-trimethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.

Biochem/physiol Actions

This antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract and various recombinant Histone proteins.

Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable