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861065

Sigma-Aldrich

Azure II

powder

Synonym(s):

3-Chloro-6-hydroxy-2-Pyridinecarboxylic acid, Azur II

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About This Item

Linear Formula:
C16H18N3S · C15H16N3S ·2 Cl
CAS Number:
Molecular Weight:
625.68
Colour Index Number:
52010/52015
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

description

mixture of Azure B and Methylene Blue in equal amounts.

Quality Level

form

powder

solubility

water: soluble

λmax

657 nm

ε (extinction coefficient)

≥1500 at 652-658 nm in water at 0.003 g/L
≥400 at 243-249 nm in water at 0.003 g/L
≥800 at 288-294 nm in water at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](\C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](\C)C

InChI

1S/C16H18N3S.C15H15N3S.2ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;/h5-10H,1-4H3;4-9H,1-3H3;2*1H/q+1;;;/p-1

InChI key

YOWZJZJLXUQHGF-UHFFFAOYSA-M

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Application

Azure II has been used in the Richardson′s stock solution for the staining of lysosomal glycogen. It has also been used in the periodic acid schiff staining procedure for the staining of glycogen, nuclei and cytoplasm.

Biochem/physiol Actions

Azure II complex is used for the staining of nuclei, goblet cells, striated muscle, endothelial cells, smooth muscle, cytoplasm, lipid droplets, red blood cells, glycogen, collagen, cartilage, mucus granules, elastin and leukocytes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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