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534730

Sigma-Aldrich

Amano Lipase from Pseudomonas fluorescens

beige-brown, ≥20,000 U/g

Synonym(s):

Lipase, triacylglycerol, Triacylglycerol lipase

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About This Item

CAS Number:
9001-62-1
EC Number:
232-619-9
MDL number:
MFCD02685888
UNSPSC Code:
12352204
NACRES:
NA.22

biological source

Pseudomonas fluorescens

specific activity

≥20,000 U/g, pH 8.0, 55 °C (Optimum pH and temperature)
≥20,000 U/g

color

beige-brown

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Application

Amano Lipase from Pseudomonas fluorescens can be used as a biocatalyst in the:
  • Hydrolysis and transesterification of parabens (para-hydroxybenzoic acid esters).
  • Enzymatic kinetic resolution of chlorohydrins.
  • Regio-selective acylation of andrographolide with different acylating agents to synthesize andrographolide derivatives.

Amano Lipase can also immobilized on silk fibroin spheres to prepare optically active epoxides by enzymatic kinetic resolution of halohydrins.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immobilization of Amano lipase from Pseudomonas fluorescens on silk fibroin spheres: an alternative protocol for the enantioselective synthesis of halohydrins
Ferreira IM, et al.
Royal Society of Chemistry Advances, 7(21), 12650-12658 (2017)
Lei Wang et al.
Journal of hazardous materials, 354, 145-152 (2018-05-12)
Hydrolysis is generally considered as the main pathway for the degradation of ester-type pollutants in aquatic environments. In this study, we found that when methanol or ethanol presented as the external carbon in the activated sludge, transesterification is very important
Lipase catalyzed synthesis of antimicrobial andrographolide derivatives
Patil Harshal S, et al.
Data in Brief, 18, 1134-1141 (2018)
Highly enantioselective acylation of chlorohydrins using Amano AK lipase from P. fluorescens immobilized on silk fibroin-alginate spheres
Ferreira IM, et al.
Tetrahedron Letters, 55(36), 5062-5065 (2014)
Dominik Koszelewski et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 25(43), 10156-10164 (2019-05-29)
A new protocol based on lipase-catalyzed tandem reaction toward α,β-enones/enoesters is presented. For the synthesis of the desired products the tandem process based on enzyme-catalyzed hydrolysis and Knoevenagel reaction starting from enol acetates and aldehyde is developed. The relevant impact
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