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Sigma-Aldrich

Williams′ Medium E

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Williams’ E medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Williams′ Medium E, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Williams′ Medium E has been shown to support the growth in long-term culture of adult liver epithelial cells.

Application

Williams′ Medium E has been used to culture primary human hepatocytes and to maintain hepatocellular carcinoma (JHH-6) cell lines.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isolation and long-term cell culture of epithelial-like cells from rat liver.
G M Williams et al.
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