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SAB5900404

Sigma-Aldrich

Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285)

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Synonym(s):

E8L, Cell surface-binding protein, Carbonic anhydrase homolog, Monkeypox virus (MPXV)

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

recombinant

expressed in CHO cells

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

clone

SAA0285, recombinant monoclonal

Assay

>95% (SDS-PAGE)

form

liquid

mol wt

150 kDa

species reactivity

monkeypox virus

concentration

1 mg/mL

technique(s)

ELISA: 1:2000-1:4000
neutralization: 0.01-10 μg/mL

color

colorless

pH

7.4

isotype

IgG2a, kappa

Protein ID accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

-65 to -96°C

target post-translational modification

unmodified

General description

Anti-Monkeypox virus/MPXV E8L Antibody, clone SAA0285, is a hybrid protein of mouse and human origin, consisting of a single light chain and a single heavy chain. The light chain is composed of a variable region (VL) and a constant region (CL) while the heavy chain is composed of a variable region (VH) and a constant region (CH). The VL and VH regions are responsible for the binding of the antibody to the antigens on the Monkeypox virus, while the CL and CH regions are responsible for the production of anti-viral proteins.
Monkeypox virus (MPXV), like smallpox virus, belongs to the genus Orthopoxvirus. Its morphology is consistent with that of orthopoxvirus, with a brick-shaped or oval shape with rounded corners and a size of 200~400nm. Brick-shaped or oval-shaped, covered by a lipoprotein membrane or envelope, with two protein-containing side bodies in the middle, and a thick membrane core containing a large double-stranded DNA gene body. The genome of Mpox virus is a double-stranded DNA, about 197kb long, and the end of the genome contains an identical but opposite terminal inverted repeat sequence. The virus contains 190 open reading frames, 4 of which are located in terminal inverted repeats. The core proteins of monkeypox virus are A29L, A35R, B6R, M1R, H3L.

Specificity

Monoclonal Anti-Monkeypox virus/MPXV E8L Antibody, clone SAA0285 binds to the antigens on the surface of the Monkeypox virus, blocking the virus from entering host cells. It also activates the production of anti-viral proteins, which neutralize the virus and inhibit its replication. The antibody also activates the immune system, helping to clear the virus from the body.

Application

ELISA,neutralization

Packaging

100µg per vial / 1 mg per vial

Storage and Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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