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SAB4200699

Sigma-Aldrich

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal

clone TH-2, purified from hybridoma cell culture

Synonym(s):

TH, TYH

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TH-2, monoclonal

form

buffered aqueous solution

species reactivity

rabbit, human, sheep, guinea pig, rat, bovine, mouse

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using whole extracts of rat PC-12 cells.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TH(7054)

Related Categories

General description

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TH-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat Tyrosine Hydroxylase. Tyrosine Hydroxylase (TH) also known as tyrosine 3- monooxygenase, is encoded by the gene mapped to human chromosome 11p15.5. It is a tetrameric enzyme composed of four subunits. Four TH Isoforms were identified in humans.

Immunogen

purified rat Tyrosine Hydroxylase

Application

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal has been used in various immunochemical techniques including immunoblotting, immunohistochemistry, immunofluorescence and immunoprecipitation.

Biochem/physiol Actions

Tyrosine Hydroxylase (TH) catalyzes the hydroxylation of L-tyrosine to L-3,4-dihydroxyphenylalanine (L-dopa) in brain and adrenal medulla which is the initial and rate limiting step of the neurotransmitters and hormones catecholamines (dopamine, norepinephrine and epinephrine) biosynthesis. Therefore, TH plays a key role in the physiology of adrenergic neurons. Tyrosine Hydroxylase (TH) deficiency is associated with the development of wide variety of phenotypic spectrum such as several types of dystonia, early-onset of Parkinson′s, cerebral palsy or spastic paraplegia and both primary and secondary deficiencies of cerebrospinal fluid (CSF) neurotransmitter metabolites.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tyrosine hydroxylase deficiency
GeneReviews(?) (2017)
Chunhui Yang et al.
Aging, 12(16), 16062-16071 (2020-08-25)
Electroconvulsive therapy (ECT) has known beneficial effects on the core motor symptoms of Parkinson's disease (PD), likely through induction of dopamine release and sensitivity of dopamine receptors. Mesenchymal stem cells (MSCs) can salvage loss of dopamine in PD through their
Tyrosine Hydroxylase and Regulation of Dopamine Synthesis
Daubner SC, et al.
Archives of Biochemistry and Biophysics, 508, 1-1 (2011)
Species differences in the expression of multiple tyrosine hydroxylase protein isoforms
Haycock J W
Journal of Neurochemistry, 81, 947-953 (2002)

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