Skip to Content
Merck
All Photos(1)

Key Documents

N2513

Sigma-Aldrich

Nerve Growth Factor β rat

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

Nerve Growth Factor-β from rat

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

biological source

rat

Quality Level

recombinant

expressed in Sf21 cells

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.15-3.00 ng/mL EC50

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: soluble 0.1 g/L at 20 °C

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... NGF(4803)

Looking for similar products? Visit Product Comparison Guide

General description

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.

Application

NGF-β from rat is used for the following applications:
  • In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
  • Western blotting analysis
  • For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
  • In cell culture and treatment (for neuronal differentiation)
  • In the preparation of stock solution

Biochem/physiol Actions

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

Analysis Note

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Guangyi Zhao et al.
Molecular medicine reports, 16(4), 4678-4684 (2017-08-30)
The present study aimed to investigate the effect of exogenous nerve growth factor (NGF) pretreatment on demyelination in the spinal cord of lidocaine‑treated rats, and explored the potential neuroprotective mechanisms of NGF. A total of 36 rats were randomly assigned
Xiao-Ping Huang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 89, 124-134 (2017-02-22)
The aim of this study was to explore the effect by which the combination of Astragaloside IV (AST IV) and Ginsenoside Rg1 (Rg1) resisted autophagic injury in PC12 cells induced by oxygen glucose deprivation/reoxygenation (OGD/R). We studied the nature of
A Khodorova et al.
Neuroscience, 254, 312-323 (2013-10-08)
Nerve growth factor (NGF) augments the excitability of isolated rat sensory neurons through activation of the p75 neurotrophin receptor (p75(NTR)) and its downstream sphingomyelin signaling cascade, wherein neutral sphingomyelinase(s) (nSMase), ceramide, and the atypical protein-kinase C (aPKC), protein-kinase M zeta
Zhe Gong et al.
Journal of neuroinflammation, 15(1), 242-242 (2018-08-30)
Nod-like receptor protein 3 (NLRP3) inflammasome is a crucial factor in mediating inflammatory responses after cerebral ischemia/reperfusion (I/R), but the cellular location of NLRP3 inflammasome in cerebral I/R has yet come to a conclusion, and there is still no specific
Saar Oz et al.
PloS one, 7(12), e51458-e51458 (2012-12-29)
Microtubules (MTs), key cytoskeletal elements in living cells, are critical for axonal transport, synaptic transmission, and maintenance of neuronal morphology. NAP (NAPVSIPQ) is a neuroprotective peptide derived from the essential activity-dependent neuroprotective protein (ADNP). In Alzheimer's disease models, NAP protects

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service