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M3534

Sigma-Aldrich

Anti-Mouse IgG (Fc specific) antibody produced in goat

2.0 mg/mL, affinity isolated antibody

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

concentration

2.0 mg/mL

technique(s)

indirect ELISA: 1:15,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Immunogen

Mouse IgG, Fc fragment

Application

Anti-Mouse IgG (Fc specific) antibody has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • Ouchterlony double diffusion
  • anti-drug antibody immunoassay
  • immunoelectrophoresis

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice

Other Notes

Antibody adsorbed with human IgG and rat serum proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human or rat samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum
Lexmond W, et al.
Journal of Immunological Methods, 373(1-2), 192-199 (2011)
Christiano M V Barbosa et al.
Cell death discovery, 5, 135-135 (2019-09-26)
Annexin A1 (AnxA1) modulates neutrophil life span and bone marrow/blood cell trafficking thorough activation of formyl-peptide receptors (FPRs). Here, we investigated the effect of exogenous AnxA1 on haematopoiesis in the mouse. Treatment of C57BL/6 mice with recombinant AnxA1 (rAnxA1) reduced
Integrating molecular detection and response to create self-signalling antibodies
Randle B, et al.
Biochemical and Biophysical Research Communications, 324(2), 504-510 (2004)
Nathan H Joh et al.
Journal of pharmaceutical sciences, 109(1), 845-853 (2019-10-20)
Silicone oil is a lubricant for prefilled syringes (PFS), a common primary container for biotherapeutics. Silicone oil particles (SiOP) shed from PFS are a concern for patients due to their potential for increased immunogenicity and therefore also of regulatory concern.
Universal immunoassay applied during early development of large molecules to understand impact of immunogenicity on biotherapeutic exposure
Bautista AC, et al.
The APPS Journal, 14(4), 843-849 (2012)

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