L3045
β-Lactamase from Pseudomonas fluorescens
lyophilized powder, recombinant, expressed in E. coli
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recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
enzyme activity
7.5-15 units/mg solid
shipped in
wet ice
storage temp.
2-8°C
Unit Definition
One unit will hydrolyze 1.0 μmole of benzylpenicillin per minute at pH 7.0 at 25 °C
Physical form
Lyophilized powder containing potassium phosphate and sodium bicarbonate
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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The Southeast Asian journal of tropical medicine and public health, 43(6), 1461-1469 (2013-02-19)
We performed extended-spectrum beta-lactamase (ESBL) phenotypic testing and molecular characterization of three ESBL genes (TEM, SHV and CTX-M) and susceptibility testing by Clinical Laboratory Standards Institute (CLSI) disk diffusion method against three cephalosporins (ceftriaxone, ceftazidime, cefepime) and a cephamycin (cefoxitin)
The Southeast Asian journal of tropical medicine and public health, 43(5), 1178-1185 (2013-02-26)
Bacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a
The Medical journal of Australia, 198(5), 267-269 (2013-03-19)
Clinical utility of carbapenem antibiotics is under threat because of the emergence of acquired metallo-β-lactamase (MBL) genes. We describe an outbreak in an intensive care unit (ICU) possibly associated with contaminated sinks. Four clusters of gram-negative bacteria harbouring the MBL
MMWR. Morbidity and mortality weekly report, 62(6), 108-108 (2013-02-15)
On August 16, 2012, the Colorado Department of Public Health and Environment was notified of two patients at an acute-care hospital in Denver with carbapenem-resistant Enterobacteriaceae (CRE), specifically Klebsiella pneumoniae (CRKP), isolated from respiratory specimens during July-August. Both isolates produced
Canadian journal of microbiology, 59(2), 110-116 (2013-03-07)
Microbial populations in wastewater treatment plants (WWTPs) are increasingly being recognized as environmental reservoirs of antibiotic resistance genes. PCR amplicons for plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS were recorded in samples from a WWTP in Vancouver, British Columbia.
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