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F8888

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:320 using human peripheral blood lymphocytes
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.
Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

Specificity

Binds chicken IgY (IgG). Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for chicken IgY (IgG).

Immunogen

Purified chicken IgY (IgG)
chicken IgY (IgG)

Application

Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody has been used in immunofluorescence.
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody may be used for immunofluorescence using human peripheral blood lymphocytes at a working antibody dilution of 1:320. For immunohistochemistry a working antibody dilution of 1:320 may be used. A dilution of 1:1000 was used for EP receptor staining of murine keratinocyte cell line 308 using primary antibodies generated in chicken. The antibody was also used for immune electron microscopy labeling of porcine nerve samples.

Biochem/physiol Actions

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens
Ingrao F, et al.
Avian Pathology : Journal of the W.V.P.A, 46(3), 289-299 (2017)
Fiona Ingrao et al.
Avian pathology : journal of the W.V.P.A, 46(3), 289-299 (2016-11-30)
Infectious bursal disease (IBD) is an avian viral disease that causes severe economic losses in the poultry industry worldwide. The live IBD virus (IBDV) has a potential immunosuppressive effect. Currently available IBDV vaccines have shortcomings, prompting the development of safer
E J Thompson et al.
Neoplasia (New York, N.Y.), 3(5), 402-410 (2001-11-01)
Recent discoveries shed light on the importance of prostaglandin (PG) production in the development of skin cancer. Work by Fischer et al. demonstrates that skin tumor promotion caused by ultraviolet B radiation can be decreased by up to 89% by
E Domínguez-Sala et al.
Brain structure & function, 227(5), 1599-1614 (2022-02-02)
Dysfunction of the LIS1 gene causes lissencephaly, a drastic neurological disorder characterized by a deep disruption of the cortical structure. We aim to uncover alterations of the cortical neuronal networks related with the propagation of epileptiform activity in the Lis1/sLis1
IgY: clues to the origins of modern antibodies
Warr G W, et al.
Immunology Today, 16(8), 392-398 (1995)

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