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F0504

Sigma-Aldrich

Folate binding protein from bovine milk

lyophilized powder

Synonym(s):

Folate Protein

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About This Item

MDL number:
MFCD00131098
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

bovine milk

form

lyophilized powder

usage

1 mg sufficient for ~4,000 assays

composition

Protein, 20-40% modified Warburg-Christian

technique(s)

ligand binding assay: suitable
surface plasmon resonance (SPR): suitable

capacity

1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.)

storage temp.

−20°C

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Application

Folate binding protein can be used in radioligand binding assays of serum folate. The folate receptor has been used in studies as a therapeutic target in a variety of cancers.
Folate binding protein from bovine milk has been used in folate binding studies using surface plasmon resonance and in the uptake studies in kidney proximal tubule cells.
Useful for radioligand binding assays of serum folate. May also bind N-methyltetrahydrofolic acid and other folate analogues.

Biochem/physiol Actions

Folate-binding protein (FBP) is present as a cell receptor and binds to folate in equimolar ratio. It is involved in the transport of folate and improves their bioavailability.

Packaging

Package size based on protein content

Quality

Purified over 1,000 fold. May contain a trace of β-mercaptoethanol.

Physical form

Lyophilized powder containing buffer salt as sodium citrate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Claire Corot et al.
Magnetic resonance in medicine, 60(6), 1337-1346 (2008-11-26)
The objective of this study was to evaluate the potential of a high-relaxivity macromolecular gadolinium (Gd) chelate to target folate receptors (FRs). P866 is a dimeric high-relaxivity Gd chelate coupled to a folate moiety. Binding affinity, in vivo biodistribution studies
A radioassay for serum folate: use of a two-phase sequential-incubation, ligand-binding system.
S P Rothenberg et al.
The New England journal of medicine, 286(25), 1335-1339 (1972-06-22)
Tumor imaging using P866, a high-relaxivity gadolinium chelate designed for folate receptor targeting
Corot C, et al.
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 60(6), 1337-1346 (2008)
Tetrahydrofolates are greatly stabilized by binding to bovine milk folate-binding protein
Jones ML and Nixon PF
The Journal of Nutrition, 132(9), 2690-2694 (2002)
The binding of folic acid and 5-methyltetrahydrofolate to folate-binding proteins during gastric passage differs in a dynamic in vitro gastrointestinal model
Verwei M, et al.
The Journal of Nutrition, 134(1), 31-37 (2004)
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