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RDD044

Sigma-Aldrich

Imidazole

ReagentPlus®, 99%, Redi-Dri™, free-flowing

Synonym(s):

Glyoxaline; 1, 3-diazole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

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About This Item

Empirical Formula (Hill Notation):
C3H4N2
CAS Number:
288-32-4
Molecular Weight:
68.08
Beilstein:
103853
MDL number:
MFCD00005183
UNSPSC Code:
12352005
NACRES:
NA.21

vapor pressure

<1 mmHg ( 20 °C)

Quality Level

100

product line

ReagentPlus®
Redi-Dri™

Assay

99%

form

powder

quality

free-flowing

pH

9-11 at 100 g/L (23 °C)

pKa (25 °C)

6.95

bp

256 °C (lit.)

mp

85-92 °C
88-91 °C (lit.)

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

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General description

Imidazole is a buffering agent employed in various research applications, with a pH 9 -11, it is often used to maintain a stable pH for protein samples. Imidazole is a heterocyclic compound with a five-membered planar ring that is amphoteric, highly polar, and commonly used for affinity chromatography. Its ability to bind to metal ions makes it particularly useful for purification and separation of proteins.

Application

Imidazole is commonly used as a buffer component for purification of the histidine-tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).
Excellent for buffers in the range of pH 6.2-7.8

Features and Benefits

  • Redi-Dri packaging controls moisture for hygroscopic chemicals that commonly clump
  • Anhydrous powder remains free-flowing and easy to use
  • Safer to handle and weigh out for routine use in lab
  • Eliminates clumps to maintain product moisture and quality

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

H302,H314,H360D

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

293.0 °F - closed cup

Flash Point(C)

145 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF1924V
WXBC8735V
WXBD9900V
WXBD9011V
WXBD2886V

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Three-Dimensional Covalent Co-Assembly between Inorganic Supertetrahedral Clusters and Imidazolates.
Wu T, et al.
Angewandte Chemie (International Edition in English), 50(11), 2536-2539 (2011)
Cobalt imidazolate framework as precursor for oxygen reduction reaction electrocatalysts.
Ma S, et al.
Chemistry?A European Journal , 17(7), 2063-2067 (2011)
Pam M Van Ry et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(8), 1285-1297 (2015-06-09)
Duchenne muscular dystrophy (DMD) is a fatal neuromuscular disease caused by mutations in the dystrophin gene, leading to the loss of a critical component of the sarcolemmal dystrophin glycoprotein complex. Galectin-1 is a small 14 kDa protein normally found in skeletal
Ali Tebbi et al.
The Journal of biological chemistry, 290(22), 14077-14090 (2015-04-17)
Ribonucleotide reductase (RnR) is a key enzyme synthesizing deoxyribonucleotides for DNA replication and repair. In mammals, the R1 catalytic subunit forms an active complex with either one of the two small subunits R2 and p53R2. Expression of R2 is S
William A Gaines et al.
Nature communications, 6, 7834-7834 (2015-07-29)
The conserved budding yeast Rad51 paralogues, including Rad55, Rad57, Csm2 and Psy3 are indispensable for homologous recombination (HR)-mediated chromosome damage repair. Rad55 and Rad57 are associated in a heterodimer, while Csm2 and Psy3 form the Shu complex with Shu1 and
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Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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