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A7409

Sigma-Aldrich

Bovine Serum Albumin solution

35% in 0.85% sodium chloride, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27
Pricing and availability is not currently available.

biological source

bovine

Quality Level

300

description

contains no preservative

sterility

aseptically filled

form

solution

purified by

heat shock fractionation

origin

USA origin

concentration

33.0-37.4% protein (biuret)
35% in 0.85% sodium chloride

technique(s)

microbiological culture: suitable

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Albumin belongs to the four-member albumin superfamily.[1] It is a major protein in the circulating blood.[2] Albumin has a helical structure with three domains that form a heart-like shape.[1]

Application

Bovine Serum Albumin solution has been used:
  • to block protein-coated well plates in enzyme-linked immunosorbent assay (ELISA) for immunoreactivity and cross-reactivity studies in the production of monoclonal antibodies (mAbs)[3]
  • to prepare primary antibody solution for peroxidases staining[4]
  • as a supplement for in vitro cultivation of Borrelia strains[4]

Biochem/physiol Actions

Bovine serum albumin (BSA) serves as a protein supplement for cell culture media.[5] BSA acts as a transport carrier for drugs, and it is commonly used to study the protein-drug interactions. Furthermore, it is known for its ability to interact with several aromatic and hydrophobic molecules of the bloodstream.[6]

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000374288
0000386367
0000384626
0000376385
0000298360

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Bovine serum albumin
Meyler's Side Effects of Drugs: The International Encyclopedia of Adverse Drug Reactions and Interactions, 1045 null
Fatai S Oladunni et al.
Journal of virological methods, 290, 114084-114084 (2021-01-30)
The use of monoclonal neutralizing antibodies (mNAbs) is being actively pursued as a viable intervention for the treatment of Severe Acute Respiratory Syndrome CoV-2 (SARS-CoV-2) infection and associated coronavirus disease 2019 (COVID-19). While highly potent mNAbs have great therapeutic potential
The NMR tube bioreactor
Methods in Enzymology, 71-101 null
Sabrina Hepner et al.
Ticks and tick-borne diseases, 10(5), 1157-1161 (2019-06-27)
Borrelia turcica is a reptile-associated Borrelia species that is vectored by the hard tick Hyalomma aegyptium. Tortoises of the genus Testudo represent the principal host of adult H. aegyptium, while immature stages are less host-specific and can be found on
Paul A Wilson et al.
PloS one, 16(10), e0258316-e0258316 (2021-10-22)
RORγt is an isoform of RORC, preferentially expressed in Th17 cells, that functions as a critical regulator of type 3 immunity. As murine Th17-driven inflammatory disease models were greatly diminished in RORC knock-out mice, this receptor was prioritised as an
View All Related Papers

Questions

  1. Is the BSA cat. A7409 suitable for MACS buffer used for T cell separation? Separated cells would be cultured for around 2 weeks

    1 answer

    1. This product has not been tested for suitability in this application. While it is aseptically prepared, this product is not tested for sterility.

      See product A7979 for the sterile, cell culture tested BSA solution that may be more suitable: https://www.sigmaaldrich.com/product/sigma/a7979

      In addition, see product A9418 to review another cell culture tested product that is cited for use in MACS buffer: https://www.sigmaaldrich.com/product/sigma/a9418

      A link to this publication is below:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059065/

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